Detects mouse Semaphorin 4C in direct ELISAs. Detects HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse Semaphorin 4C Antibody

Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) mouse Semaphorin 4C or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Semaphorin 4C Monoclonal Antibody (Catalog # MAB8497) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005).

Applications for Mouse Semaphorin 4C Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4C

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.

Alternate Names

SEMA4C, SEMACL1, Semaf, SEMAI

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Gene Symbol

SEMA4C

UniProt

Q64151

Additional Semaphorin 4C Products

Product Documents for Mouse Semaphorin 4C Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Semaphorin 4C Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Semaphorin 4C Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8497

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Semaphorin 4C Antibodies »

Product Specifications

Immunogen

Specificity