Mouse Semaphorin 4D/CD100 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5235
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
Phe24-Thr657
Accession # NP_038688
Phe24-Thr657
Accession # NP_038688
Specificity
Detects mouse Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 4B, and rhSemaphrin 6C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Semaphorin 4D/CD100 Antibody
Detection of Semaphorin 4D/ CD100 in EL‑4 Mouse Cell Line by Flow Cytometry.
EL-4 mouse lymphoblast cell line were stained with Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5235, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Semaphorin 4D/CD100 in Mouse Embryo Tissue.
Semaphorin 4D/CD100 was detected in immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.) using Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5235) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Semaphorin 4D/CD100 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: EL-4 mouse lymphoblast cell line
Sample: EL-4 mouse lymphoblast cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Semaphorin 4D/CD100
Sample: Recombinant Mouse Semaphorin 4D/CD100
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4D/CD100
Alternate Names
A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ
Gene Symbol
SEMA4D
UniProt
Additional Semaphorin 4D/CD100 Products
Product Documents for Mouse Semaphorin 4D/CD100 Antibody
Product Specific Notices for Mouse Semaphorin 4D/CD100 Antibody
For research use only
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