Mouse Serpin A8/Angiotensinogen Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6966
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 754438
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
Asp25-Val477
Accession # P11859
Asp25-Val477
Accession # P11859
Specificity
Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots.
In Western blots, no cross-reactivity
with recombinant human (rh) Serpin A8/Angiotensinogen, rhSerpin A4, recombinant
mouse (rm) Serpin A1, A3N, A11, B8, C1, E1, E2, I1, or rmPEDF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Serpin A8/Angiotensinogen Antibody
Detection of Mouse Serpin A8/Angiotensinogen by Western Blot.
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Serpin A8/Angiotensinogen at approximately 52-62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin A8/Angiotensinogen in Mouse Intestine.
Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Serpin A8/Angiotensinogen Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine
Sample: Perfusion fixed frozen sections of mouse intestine
Western Blot
0.5 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A8/Angiotensinogen
Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human Serpin A8 is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Serpin A8 may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human Serpin A8 shares 61% aa identity with mouse Serpin A8.
Alternate Names
AGT, Angiotensin II, Angiotensinogen, Aogen, PAT
Gene Symbol
AGT
UniProt
Additional Serpin A8/Angiotensinogen Products
Product Documents for Mouse Serpin A8/Angiotensinogen Antibody
Product Specific Notices for Mouse Serpin A8/Angiotensinogen Antibody
For research use only
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