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Mouse Serpin B8/Proteinase Inhibitor 8 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3588

R&D Systems, part of Bio-Techne
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AF3588
AF3588-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # O08800

Specificity

Detects mouse Serpin B8/Proteinase Inhibitor 8 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Serpin B8, rhSerpin B9, and rhSerpin B6 is observed and less than 1% cross-reactivity with rhSerpin A1, D1, E2, I2, recombinant mouse Serpin A1, C1, E1, F1, and F2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Serpin B8/Proteinase Inhibitor 8 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin B8/Proteinase Inhibitor 8, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Serpin B8/Proteinase Inhibitor 8

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B8/Proteinase Inhibitor 8

Serpin B8, also known as Proteinase Inhibitor 8 (PI8) and cytoplasmic antiproteinase 2 (CAP2), is a member of the Serpin superfamily of the serine protease inhibitors (1). It inhibits furin, a ubiquitously expressed convertase that processes many pro-proteins (2). Distribution of Serpin B8 alters during kidney regeneration, indicating that it plays a role in inflammation or tissue repair (3).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Dahlen, J.R. et al. (1998) J. Biol. Chem. 273:1851.
  3. Gillard, A. (2006) Am. J. Nephrol. 26:34.
  4. .

Alternate Names

CAP2, NK10, PI8

Entrez Gene IDs

5271 (Human); 20725 (Mouse); 288937 (Rat)

Gene Symbol

SERPINB8

UniProt

Additional Serpin B8/Proteinase Inhibitor 8 Products

Product Documents for Mouse Serpin B8/Proteinase Inhibitor 8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Serpin B8/Proteinase Inhibitor 8 Antibody

For research use only

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