Mouse SEZ6/BSRP-C Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4989
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SEZ6/BSRP-C
Gly19-Asn902
Accession # Q7TSK2
Gly19-Asn902
Accession # Q7TSK2
Specificity
Detects mouse SEZ6/BSRP-C in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse (rm) BSRP-A and rmBSRP-B is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse SEZ6/BSRP-C Antibody
SEZ6/BSRP-C in Mouse Brain.
SEZ6/BSRP-C was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6/BSRP-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4989) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies, processes, and synaptic puncta. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse SEZ6/BSRP-C Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse SEZ6/BSRP-C
Sample: Recombinant Mouse SEZ6/BSRP-C
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SEZ6/BSRP-C
SEZ6 (Seizure protein 6, also BSRP-C, Brain-specific receptor-like protein C) is a 150 kDa member of the BSRP family of proteins. It is expressed on neurons and exerts an antibranching effect relative to neurite extension. Mature mouse SEZ6 is a 974 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular domain (ECD) of at least 876 amino acids (aa 18‑893). The ECD shows three CUB domains and five short consensus repeats (SCRs). There are two splice variants for SEZ6. One is soluble, and shows an 18 aa substitution for aa 588‑991, while a second shows a deletion of aa 895‑907 plus a 9 aa substitution for aa 982‑991. Over aa 19‑902, mouse SEZ6 is 91% aa identical to human SEZ6.
Long Name
Seizure Related 6/Brain-specific Receptor-like Protein-C
Alternate Names
BSRP-C, BSRPC
Gene Symbol
SEZ6
UniProt
Additional SEZ6/BSRP-C Products
Product Documents for Mouse SEZ6/BSRP-C Antibody
Product Specific Notices for Mouse SEZ6/BSRP-C Antibody
For research use only
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