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Mouse SEZ6L2/BSRP-A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4916

R&D Systems, part of Bio-Techne
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MAB4916
MAB4916-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 529805

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
Leu28-Ser837
Accession # Q4V9Z5

Specificity

Detects mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) BSRP‑B or rmBSRP‑C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse SEZ6L2/BSRP-A Antibody

SEZ6L2/BSRP-A antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

SEZ6L2/BSRP-A in Mouse Brain.

SEZ6L2/BSRP-A was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using 25 µg/mL Rat Anti-Mouse SEZ6L2/BSRP-A Monoclonal Antibody (Catalog # MAB4916) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse SEZ6L2/BSRP-A antibody by Western Blot.

Detection of Mouse SEZ6L2/BSRP-A by Western Blot.

Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SEZ6L2/BSRP-A Monoclonal Antibody (Catalog # MAB4916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SEZ6L2/BSRP-A at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse SEZ6L2/BSRP-A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)

Western Blot

2 µg/mL
Sample: Mouse brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEZ6L2/BSRP-A

BSRP-A (Brain-specific receptor-like protein A; also known as SEZ6L2) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, and likely regulates PKC alpha activation. Mature mouse BSRP-A is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for BSRP-A. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse BSRP-A shares 96% and 99% aa sequence identity with human and rat BSRP-A, respectively.

Long Name

Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A

Alternate Names

BSRP-A, BSRPA, PSK-1

Entrez Gene IDs

26470 (Human); 233878 (Mouse); 308988 (Rat)

Gene Symbol

SEZ6L2

UniProt

Additional SEZ6L2/BSRP-A Products

Product Documents for Mouse SEZ6L2/BSRP-A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse SEZ6L2/BSRP-A Antibody

For research use only

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