Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec‑1/CD169, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Siglec-1/CD169 PE-conjugated Antibody

Detection of Siglec‑1/CD169 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight were stained with Rat Anti-Mouse Siglec-1/CD169 PE-conjugated Monoclonal Antibody (Catalog # FAB5610P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Siglec-1/CD169 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB5610P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-1/CD169

Siglec-1, also known as Sialoadhesin or CD169, is a 175-185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human); 20612 (Mouse); 311426 (Rat)

Gene Symbol

SIGLEC1

UniProt

Q62230

Additional Siglec-1/CD169 Products

Product Documents for Mouse Siglec-1/CD169 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Siglec-1/CD169 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse Siglec-1/CD169 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5610P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications