Detects mouse TLR4 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse TLR4 PE-conjugated Antibody

Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

LPS-activated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR4 PE-conjugated Monoclonal Antibody (Catalog # FAB2759P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TLR4 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: LPS-activated RAW 264.7 mouse monocyte/macrophage cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name

Toll-like Receptor 4

Alternate Names

CD284

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Gene Symbol

TLR4

UniProt

Q9QUK6

Additional TLR4 Products

Product Documents for Mouse TLR4 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TLR4 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse TLR4 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2759P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications