Mouse TORC3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6840

R&D Systems, part of Bio-Techne
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MAB6840
MAB6840-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 643729

View all TORC3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TORC3
Leu153-Pro275
Accession # Q91X84

Specificity

Detects mouse TORC3 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human TORC3 and no cross-reactivity with recombinant mouse (rm) TORC1 or rmTORC2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TORC3 Antibody

Detection of Mouse TORC3 antibody by Western Blot.

Detection of Mouse TORC3 by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TORC3 Monoclonal Antibody (Catalog # MAB6840) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TORC3 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse TORC3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TORC3

TORC3, also known as CREB-regulated transcription coactivator 1 (crtc3), is an approximately 80 kDa protein that functions as a coactivator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC3 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1 transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1 transcription. Alternate splicing of mouse TORC3 generates isoforms that lack the N-terminal 299 amino acids (aa). Within aa 153-275, mouse TORC3 shares 99% and 98% aa sequence identity with human and rat TORC3, respectively.

Long Name

Transducer of Regulated CREB-binding Protein 3

Alternate Names

CRTC3

Entrez Gene IDs

64784 (Human); 70461 (Mouse); 365297 (Rat)

Gene Symbol

CRTC3

UniProt

Q91X84

Additional TORC3 Products

Product Documents for Mouse TORC3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse TORC3 Antibody

For research use only

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