CD8⁺CD11c⁺mouse splenocytes were stained with Rat Anti-Mouse TREML4/TLT-4 Monoclonal Antibody (Catalog # MAB5500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse TREML4/TLT-4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: CD8⁺ CD11c⁺ mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML4/TLT-4

TREML4 (Triggering Receptor Expressed on Myeloid cells-Like 4), also known as TLT4, is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue. There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 4

Alternate Names

TLT4

Entrez Gene IDs

285852 (Human); 224840 (Mouse); 680862 (Rat)

Gene Symbol

TREML4

UniProt

Q3LRV9

Additional TREML4/TLT-4 Products

Product Documents for Mouse TREML4/TLT-4 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TREML4/TLT-4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TREML4/TLT-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5500

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TREML4/TLT-4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TREML4/TLT-4 Antibody

Detection of TREML4 in Mouse Splenocytes by Flow Cytometry.