Mouse TREML4/TLT-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5500
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT‑4
Ser25-Val195
Accession # NP_001029094
Ser25-Val195
Accession # NP_001029094
Specificity
Detects mouse TREML4/TLT‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREM1, rmTREM2b, and rmTREM3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse TREML4/TLT-4 Antibody
Detection of Mouse TREML4/TLT-4 by Western Blot.
Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse TREML4/TLT-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5500) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TREML4/TLT-4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse TREML4/TLT-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse spleen tissue
Sample: Mouse spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREML4/TLT-4
TREML4 (Triggering receptor expressed on myeloid cells-like
4; also TLT4) is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and
based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane
(TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa
extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the
TM segment contains one
Long Name
Triggering Receptor Expressed on Myeloid Cells-like 4
Alternate Names
TLT4
Gene Symbol
TREML4
UniProt
Additional TREML4/TLT-4 Products
Product Documents for Mouse TREML4/TLT-4 Antibody
Product Specific Notices for Mouse TREML4/TLT-4 Antibody
For research use only
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