Mouse TSLP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF555
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6
Tyr20-Glu140
Accession # Q9JIE6
Specificity
Detects mouse TSLP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse TSLP Antibody
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.
Recombinant Mouse TSLP (555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND50 is typically ≤0.4 µg/mL.
Detection of Mouse TSLP by Immunohistochemistry-Paraffin
Klk5 deletion results in remarkable suppression of the inflammatory phenotype of NS.(A-E). Results show remarkable abrogation of pro-allergic and pro-inflammatory cytokines quantified by RT-qPCR. (A) Il-1 beta, Tslp, Tnf-alpha ; (B) Il-6, Il-18, the type 17 promoting cytokine Il-23p19; (C) Ifn-gamma, Il-4, Il-17A, Il-22; (D) Th17 regulated genes Defb4, Slpi, Cxcl1 and Ccl20; (E) Th17/22 regulated genes s100a7, s100a8, s100a9. Data are shown as the mean ± s.e.m. of triplicate amplification for at least five mice per genotype. Results are normalized to wt mean (set as 1.0). *p<0.05, **p<0.005, ***p<0.001 (Mann-Whitney U test). (F) Quantification of mast cells based on toluidine blue staining of skin tissue (mast cells stain purple). An increased number of mast cells was detected in Spink5-/- dermis; (G) IHC analysis shows abolishment of Tslp expression in Spink5-/-Klk5-/- skin as compared to Spink5-/-; Scale bar: 50μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26390218), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TSLP by Immunohistochemistry-Paraffin
Klk5 deletion results in remarkable suppression of the inflammatory phenotype of NS.(A-E). Results show remarkable abrogation of pro-allergic and pro-inflammatory cytokines quantified by RT-qPCR. (A) Il-1 beta, Tslp, Tnf-alpha ; (B) Il-6, Il-18, the type 17 promoting cytokine Il-23p19; (C) Ifn-gamma, Il-4, Il-17A, Il-22; (D) Th17 regulated genes Defb4, Slpi, Cxcl1 and Ccl20; (E) Th17/22 regulated genes s100a7, s100a8, s100a9. Data are shown as the mean ± s.e.m. of triplicate amplification for at least five mice per genotype. Results are normalized to wt mean (set as 1.0). *p<0.05, **p<0.005, ***p<0.001 (Mann-Whitney U test). (F) Quantification of mast cells based on toluidine blue staining of skin tissue (mast cells stain purple). An increased number of mast cells was detected in Spink5-/- dermis; (G) IHC analysis shows abolishment of Tslp expression in Spink5-/-Klk5-/- skin as compared to Spink5-/-; Scale bar: 50μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26390218), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse TSLP Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TSLP (Catalog # 555-TS)
Sample: Recombinant Mouse TSLP (Catalog # 555-TS)
Neutralization
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha/CD127 [Park, L.S. et al. (2000) J. Exp. Med. 192:659]. The Neutralization Dose (ND50) is typically ≤0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP
Stromal Lymphopoietin (TSLP) was originally identified from the conditioned medium of a mouse thymic stromal cell line as a protein that promoted the development of B cells. The activity of mouse TSLP overlaps with, but is distinct from, that of mouse IL-7 (1). Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites. The Sf 21 cell expressed rmTSLP is likely to be glycosylated at all three sites, as three major glycoforms were visible on SDS-PAGE (Figure 1). Insect cells are known to express relatively simple and homogeneous N-glycans that mainly belong to the high mannose type (2). This recombinant protein was found to be an excellent substrate for N-specific glycosidases such as Endo F3 (Figure 1). The majority of the glycans on rmTSLP can be readily removed by Endo F3. However, a small percentage of the glycans is somewhat resistant to Endo F3 digestion, possibly lacking core fucose, as it is known that core fucosylated N-glycans are strongly preferred substrates for Endo F3 digestion (3).
References
- Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
- Staudacher, E. et al. (1992) Eur. J. Biochem. 207:987.
- Tarentino, A.L. and T.H. Jr. Plummer (1994) Glycobiology 4: 771.
Long Name
Thymic Stromal Lymphopoietin
Alternate Names
thymic stromal lymphopoietin
Gene Symbol
TSLP
UniProt
Additional TSLP Products
Product Documents for Mouse TSLP Antibody
Product Specific Notices for Mouse TSLP Antibody
For research use only
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