Mouse VSIG2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4766
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 771625
Product Summary for Mouse VSIG2 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VSIG2
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109
Specificity
Detects mouse VSIG2 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human VSIG2 or recombinant mouse VSIG1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse VSIG2 Antibody
VSIG2 in Mouse Stomach.
VSIG2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse VSIG2 Monoclonal Antibody (Catalog # MAB4766) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the plasma membranes of cells in gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse VSIG2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse stomach
Sample: Perfusion fixed frozen sections of mouse stomach
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG2
VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236.
Long Name
V-Set and Immunoglobulin Domain Containing 2
Alternate Names
CTH, CTXL
Gene Symbol
VSIG2
UniProt
Additional VSIG2 Products
Product Documents for Mouse VSIG2 Antibody
Product Specific Notices for Mouse VSIG2 Antibody
For research use only
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