Mouse VSIG2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4766
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VSIG2
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109
Specificity
Detects mouse VSIG2 in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) VSIG2 is observed and less than 10% cross-reactivity with rhVSIG3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse VSIG2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse VSIG2
Sample: Recombinant Mouse VSIG2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG2
VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236.
Long Name
V-Set and Immunoglobulin Domain Containing 2
Alternate Names
CTH, CTXL
Gene Symbol
VSIG2
UniProt
Additional VSIG2 Products
Product Documents for Mouse VSIG2 Antibody
Product Specific Notices for Mouse VSIG2 Antibody
For research use only
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