Mouse WIF-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB135
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 134714
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse WIF-1
Gly29-Trp379
Accession # Q9WUA1
Gly29-Trp379
Accession # Q9WUA1
Specificity
Detects mouse WIF-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human WIF-1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse WIF-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse WIF-1
Sample: Recombinant Mouse WIF-1
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WIF-1
WIF-1 is a secreted protein of approximately 50 kDa that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Mature mouse WIF-1 shares 95% and 98% amino acid sequence identity with human and rat WIF-1, respectively.
Long Name
Wnt Inhibitory Factor
Alternate Names
WIF1
Gene Symbol
WIF1
UniProt
Additional WIF-1 Products
Product Documents for Mouse WIF-1 Antibody
Product Specific Notices for Mouse WIF-1 Antibody
For research use only
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