Mouse Wnt-11 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3746
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 310622
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-11
Leu39-Ala79 and Ser225-Arg297
Accession # NP_033545
Leu39-Ala79 and Ser225-Arg297
Accession # NP_033545
Specificity
Detects mouse Wnt-11 in direct ELISAs and Western blots. In these formats, this antibody shows no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or –9a. Western blotting with human cell extracts demonstrates that this antibody also detects human Wnt-11.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Wnt-11 Antibody
Detection of Mouse Wnt‑11 by Western Blot.
Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Wnt‑11 in Mouse Embryo.
Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt-11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Wnt-11 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)
Western Blot
2 µg/mL
Sample: Mouse embryo (13 d.p.c.) tissue
Sample: Mouse embryo (13 d.p.c.) tissue
Reviewed Applications
Read 2 reviews rated 4.5 using MAB3746 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-11
Long Name
Wingless-type MMTV Integration Site Family, Member 11
Alternate Names
Wnt11
Gene Symbol
WNT11
UniProt
Additional Wnt-11 Products
Product Documents for Mouse Wnt-11 Antibody
Product Specific Notices for Mouse Wnt-11 Antibody
For research use only
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