Mouse Wnt-8b Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB33671
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 341502
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Specificity
Detects mouse Wnt-8b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b,-9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Wnt-8b Antibody
Detection of Mouse Wnt-8b by Western Blot.
Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt-8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.Applications for Mouse Wnt-8b Antibody
Application
Recommended Usage
Western Blot
5 µg/mL
Sample: KATO-III human gastric carcinoma cell line
Sample: KATO-III human gastric carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-8b
Long Name
Wingless-type MMTV Integration Site Family, Member 8b
Alternate Names
Wnt8b
Gene Symbol
WNT8B
UniProt
Additional Wnt-8b Products
Product Documents for Mouse Wnt-8b Antibody
Product Specific Notices for Mouse Wnt-8b Antibody
For research use only
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