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MP1/MAP2K1IP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50631

Novus Biologicals, part of Bio-Techne
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NBP1-50631
NBP1-50631SS

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.08 mg/ml

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]

Localization

Late endosome membrane; Peripheral membrane protein; Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MP1/MAP2K1IP1 Antibody - BSA Free

Western Blot: MP1/MAP2K1IP1 AntibodyBSA Free [NBP1-50631]

Western Blot: MP1/MAP2K1IP1 AntibodyBSA Free [NBP1-50631]

Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: MP1/MAP2K1IP1 Antibody - BSA Free [NBP1-50631]

Immunocytochemistry/ Immunofluorescence: MP1/MAP2K1IP1 Antibody - BSA Free [NBP1-50631]

Immunocytochemistry/Immunofluorescence: MP1/MAP2K1IP1 Antibody [NBP1-50631] - ICC staining of MAP2K1IP1 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MP1/MAP2K1IP1 Antibody - BSA Free [NBP1-50631]

Immunohistochemistry: MP1/MAP2K1IP1 Antibody - BSA Free [NBP1-50631]

Immunohistochemistry: MP1/MAP2K1IP1 Antibody [NBP1-50631] - IHC staining of MAP2K1IP1 in mouse lung.

Applications for MP1/MAP2K1IP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Simple Western

1:40

Western Blot

1:2000
Application Notes
This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.08 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MP1

Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.

Long Name

MEK-binding Protein 1

Alternate Names

LAMTOR3, MAP2K1IP1, Mapbp

Entrez Gene IDs

8649 (Human); 56692 (Mouse)

Gene Symbol

LAMTOR3

Additional MP1 Products

Product Documents for MP1/MAP2K1IP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MP1/MAP2K1IP1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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