MPP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85038

Novus Biologicals, part of Bio-Techne
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NBP1-85038
NBP1-85038-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MPP2 Antibodies »

Product Summary for MPP2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LRAMNRAALESGISTKQLTEADLRRTVEESSRIQRGYGHYFDLCLVNSNLERTFRELQT

Localization

Mitochondria

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MPP2 Antibody

Western Blot: MPP2 Antibody [NBP1-85038]

Western Blot: MPP2 Antibody [NBP1-85038]

Western Blot: MPP2 Antibody [NBP1-85038] - Analysis in control (vector only transfected HEK293T lysate) and MPP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MPP2 Antibody [NBP1-85038]

Immunocytochemistry/ Immunofluorescence: MPP2 Antibody [NBP1-85038]

Immunocytochemistry/Immunofluorescence: MPP2 Antibody [NBP1-85038] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: MPP2 Antibody [NBP1-85038]

Immunohistochemistry-Paraffin: MPP2 Antibody [NBP1-85038]

Immunohistochemistry-Paraffin: MPP2 Antibody [NBP1-85038] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Applications for MPP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MPP2

MPP2 (Palmitoylated membrane protein 2) is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP2/Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.

Alternate Names

Discs large homolog 2, DKFZp686A06252, DKFZp686J2189, DKFZp761D0712, DLG2discs large, homolog 2, MAGUK p55 subfamily member 2, membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2), Protein MPP2

Gene Symbol

MPP2

Additional MPP2 Products

Product Documents for MPP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MPP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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