MPP3 Antibody (4D7)

Novus Biologicals, part of Bio-Techne | Catalog # H00004356-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MPP3 Antibodies »

Product Specifications

Immunogen

MPP3 (AAH56865, 1 a.a. ~ 585 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPVLSEDSGLHETLALLTSQLRPDSNHKEEMGFLRDVFSEKSLSYLMKIHEKLRYYERQSPTPVLHSAVALAEDVMEELQAASVHSDERELLQLLSTPHLRAVLMVHDTVAQKNFDPVLPPLPDNIDEDFDEESVKIVRLVKNKEPLGATIRRDEHSGAVVVARIMRGGAADRSGLVHVGDELREVNGIAVLHKRPDEISQILAQSQGSITLKIIPATQEEDRLKESKVFMRALFHYNPREDRAIPCQEAGLPFQRRQVLEVVSQDDPTWWQAKRVGDTNLRAGLIPSKGFQERRLSYRRAAGTLPSPQSLRKPPYDQPCDKETCDCEGYLKGHYVAGLRRSFRLGCRERLGGSQEGKMSSGAESPELLTYEEVARYQHQPGERPRLVVLIGSLGARLHELKQKVVAENPQHFGVAVPHTTRPRKSHEKEGVEYHFVSKQAFEADLHHNKFLEHGEYKENLYGTSLEAIQAVMAKNKVCLVDVEPEALKQLRTSEFKPYIIFVKPAIQEKRKTPPMSPACEDTAAPFDEQQQEMAASAAFIDRHYGHLVDAVLVKEDLQGAYSQLKVVLEKLSKDTHWVPVSWVR

Specificity

MPP3 - membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MPP3 Antibody (4D7)

Western Blot: MPP3 Antibody (4D7) [H00004356-M01]

Western Blot: MPP3 Antibody (4D7) [H00004356-M01]

Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.Lane 1: MPP3 transfected lysate(66.2 KDa).Lane 2: Non-transfected lysate.
Western Blot: MPP3 Antibody (4D7) [H00004356-M01]

Western Blot: MPP3 Antibody (4D7) [H00004356-M01]

Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # H00004356-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: MPP3 Antibody (4D7) [H00004356-M01] - Detection limit for recombinant GST tagged MPP3 is approximately 0.03ng/ml as a capture antibody.

Applications for MPP3 Antibody (4D7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MPP3

This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.

Alternate Names

Discs large homolog 3, discs, large homolog 3, DLG3MAGUK p55 subfamily member 3, membrane protein palmitoylated 3, membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3), Protein MPP3

Entrez Gene IDs

4356 (Human)

Gene Symbol

MPP3

OMIM

601114 (Human)

UniProt

AAH56865 (Human)

Additional MPP3 Products

Product Documents for MPP3 Antibody (4D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MPP3 Antibody (4D7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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