MPPE1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86089
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PEVKTTASDGEQTTREPVLKAMFLADTHLLGEFLGHWLDKLRREWQMERAFQTALWLLQPEVVFILGDIFDEGKWSTPEAWADDVERFQKMFRHPSHVQLKVVAGNHDIGFHYEMN
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MPPE1 Antibody
Western Blot: MPPE1 Antibody [NBP1-86089]
Western Blot: MPPE1 Antibody [NBP1-86089] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissueImmunocytochemistry/ Immunofluorescence: MPPE1 Antibody [NBP1-86089]
Immunocytochemistry/Immunofluorescence: MPPE1 Antibody [NBP1-86089] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.Immunohistochemistry-Paraffin: MPPE1 Antibody [NBP1-86089]
Immunohistochemistry-Paraffin: MPPE1 Antibody [NBP1-86089] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.Applications for MPPE1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MPPE1
Alternate Names
EC 3.1, metallo phosphoesterase, metallophosphoesterase 1, PGAP5, Post-GPI attachment to proteins factor 5
Gene Symbol
MPPE1
Additional MPPE1 Products
Product Documents for MPPE1 Antibody
Product Specific Notices for MPPE1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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