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MPPE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86089

Novus Biologicals, part of Bio-Techne
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NBP1-86089
NBP1-86089-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PEVKTTASDGEQTTREPVLKAMFLADTHLLGEFLGHWLDKLRREWQMERAFQTALWLLQPEVVFILGDIFDEGKWSTPEAWADDVERFQKMFRHPSHVQLKVVAGNHDIGFHYEMN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MPPE1 Antibody

Western Blot: MPPE1 Antibody [NBP1-86089]

Western Blot: MPPE1 Antibody [NBP1-86089]

Western Blot: MPPE1 Antibody [NBP1-86089] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: MPPE1 Antibody [NBP1-86089]

Immunocytochemistry/ Immunofluorescence: MPPE1 Antibody [NBP1-86089]

Immunocytochemistry/Immunofluorescence: MPPE1 Antibody [NBP1-86089] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MPPE1 Antibody [NBP1-86089]

Immunohistochemistry-Paraffin: MPPE1 Antibody [NBP1-86089]

Immunohistochemistry-Paraffin: MPPE1 Antibody [NBP1-86089] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for MPPE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MPPE1

MPPE1( AAH02877, 34 a.a. - 334 a.a.) full-length recombinant protein with GST.

Alternate Names

EC 3.1, metallo phosphoesterase, metallophosphoesterase 1, PGAP5, Post-GPI attachment to proteins factor 5

Gene Symbol

MPPE1

Additional MPPE1 Products

Product Documents for MPPE1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MPPE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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