MRCL3 [p Ser18] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05425

Novus Biologicals, part of Bio-Techne
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NBP3-05425
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MRCL3 Antibodies »

Product Specifications

Immunogen

A phospho specific peptide corresponding to residues surrounding S18 of human MRCL3.

Modification

p Ser18

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRCL3 [p Ser18] Antibody - BSA Free

Western Blot: MRCL3 [p Ser18] Antibody [NBP3-05425] - Western blot analysis of extracts from 293 cells untreated or treated with starvation using MRCL3 antibody (NBP3-05425). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunohistochemistry-Paraffin: MRCL3 [p Ser18] Antibody - BSA Free [NBP3-05425]

Immunohistochemistry-Paraffin: MRCL3 [p Ser18] Antibody - BSA Free [NBP3-05425]

Immunohistochemistry-Paraffin: MRCL3 [p Ser18] Antibody [NBP3-05425] - Immunohistochemistry of paraffin-embedded human breast carcinoma using MRCL3 antibody (NBP3-05425). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for MRCL3 [p Ser18] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRCL3

The muscle myosins and hexomeric proteins consisting of two heavy chains and two pairs of light chains, the latter called essential (ELC) and regulatory (RLC). The light chains stabilize the long alpha helical neck of the myosin head (1). Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (2). Familial hypertrophic cardiomyopathy (FHC) is an autosomal dominant disease caused by mutations in all of the major sarcomeric proteins, including the ventricular myosin regulatory light-chain (RLC). The Myosin RLC mutation has been associated with a rare variant of cardiac hypertrophy defined by mid-left ventricular obstruction due to papillary muscle hypertrophy. This mutation was later found to cause ventricular and septal hypertrophy (3).

Alternate Names

MLCBMyosin RLC, MRCL3, MRLC3MLC-2B, MYL2B, myosin regulatory light chain 12A, Myosin regulatory light chain 2, nonsarcomeric, myosin regulatory light chain 3, myosin regulatory light chain MRCL3, Myosin regulatory light chain MRLC3, myosin, light chain 12A, regulatory, non-sarcomeric, myosin, light polypeptide, regulatory, non-sarcomeric (20kD), RLC

Gene Symbol

MYL12A

Additional MRCL3 Products

Product Documents for MRCL3 [p Ser18] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MRCL3 [p Ser18] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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