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MRP4/ABCC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1471

Novus Biologicals, part of Bio-Techne
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NB100-1471

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence CNGQPSTLTIFETAL corresponding to C-Terminus according to NP_005836.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28785115). Rat reactivity reported in scientific literature (PMID: 22419695).

Localization

Cytoplasm

Specificity

This antibody is expected to recognize isoform 1 (NP_005836.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MRP4/ABCC4 Antibody

Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471]

Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471]

Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for MRP4/ABCC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:1000

Western Blot

1-3 ug/ml
Application Notes
ICC/IF successful usage for human primary hepatocytes is reported in scientific literature (PMID: 20395535). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31669256).WB reactivity reported in (PMID: 25263207). Data obtained from a previous batch (different goat). WB: Approx. 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa band according to rat NP_596902.1 and 150 kDa band according to human NP_005836.2) is provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Data obtained from a previous batch (different goat). IHC-P: Human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRP4/ABCC4

MRP4 may be an organic anion pump relevant to cellular detoxification

Long Name

Multidrug Resistance-associated Protein 4

Alternate Names

ABCC4, MOATB

Entrez Gene IDs

10257 (Human); 170924 (Rat)

Gene Symbol

ABCC4

UniProt

Additional MRP4/ABCC4 Products

Product Documents for MRP4/ABCC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRP4/ABCC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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