MRP4/ABCC4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1471
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CNGQPSTLTIFETAL corresponding to C-Terminus according to NP_005836.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28785115). Rat reactivity reported in scientific literature (PMID: 22419695).
Localization
Cytoplasm
Specificity
This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MRP4/ABCC4 Antibody
Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471]
Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for MRP4/ABCC4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
4 - 6 ug/mL
Immunohistochemistry-Paraffin
4 - 6 ug/mL
Peptide ELISA
Detection limit 1:1000
Western Blot
1-3 ug/ml
Application Notes
ICC/IF successful usage for human primary hepatocytes is reported in scientific literature (PMID: 20395535). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31669256).WB reactivity reported in (PMID: 25263207). Data obtained from a previous batch (different goat). WB: Approx. 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa band according to rat NP_596902.1 and 150 kDa band according to human NP_005836.2) is provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Data obtained from a previous batch (different goat). IHC-P: Human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MRP4/ABCC4
Long Name
Multidrug Resistance-associated Protein 4
Alternate Names
ABCC4, MOATB
Gene Symbol
ABCC4
UniProt
Additional MRP4/ABCC4 Products
Product Documents for MRP4/ABCC4 Antibody
Product Specific Notices for MRP4/ABCC4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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