MRPL12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19397

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-19397
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MRPL12 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRPL12 Antibody

Western Blot: MRPL12 Antibody [NBP2-19397]

Western Blot: MRPL12 Antibody [NBP2-19397]

Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397]

Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397]

Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
MRPL12 Antibody

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody [NBP2-19397] -

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody [NBP2-19397] - MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:500.
Blue: Hoechst 33342 staining.
View 1 more images

Applications for MRPL12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MRPL12

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]

Alternate Names

5c5-2, FLJ60124, L12mt, MGC8610, mitochondrial ribosomal protein L12, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML1239S ribosomal protein L12, mitochondrial

Gene Symbol

MRPL12

UniProt

P52815 (Human)

Additional MRPL12 Products

Product Documents for MRPL12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MRPL12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.