MRPL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-863

Novus Biologicals, part of Bio-Techne
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NB300-863
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MRPL3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence NVCQPGAPSITFA corresponding to C-Terminus according to NP_009139.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MRPL3 Antibody

Western Blot: MRPL3 Antibody [NB300-863]

Western Blot: MRPL3 Antibody [NB300-863]

Western Blot: MRPL3 Antibody [NB300-863] (1ug/ml) of HepG2 cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for MRPL3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 38.6 kDa band according to NP_009139.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRPL3

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q.

Alternate Names

39S ribosomal protein L3, mitochondrial, mitochondrial ribosomal protein L3, MRL3mitochondrial, L3, RPML3

Entrez Gene IDs

11222 (Human)

Gene Symbol

MRPL3

UniProt

NP_009139. (Human)

Additional MRPL3 Products

Product Documents for MRPL3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MRPL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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