MRPL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19404

Novus Biologicals, part of Bio-Techne
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NBP2-19404
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Primate (96%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MRPL3 Antibodies »

Product Summary for MRPL3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL3. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (96%), Bovine (87%), Rat (89%), Xenopus laevis (80%), Chicken (80%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRPL3 Antibody

MRPL3 Antibody

Western Blot: MRPL3 Antibody [NBP2-19404] -

Western Blot: MRPL3 Antibody [NBP2-19404] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MRPL3 antibody [N3C3] (NBP2-19404) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MRPL3 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MRPL3

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q. [provided by RefSeq]

Alternate Names

39S ribosomal protein L3, mitochondrial, mitochondrial ribosomal protein L3, MRL3mitochondrial, L3, RPML3

Gene Symbol

MRPL3

UniProt

P09001 (Human)

Additional MRPL3 Products

Product Documents for MRPL3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MRPL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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