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MRPS10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19409

Novus Biologicals, part of Bio-Techne
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NBP2-19409

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPS10. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRPS10 Antibody

Western Blot: MRPS10 Antibody [NBP2-19409]

Western Blot: MRPS10 Antibody [NBP2-19409]

Western Blot: MRPS10 Antibody [NBP2-19409] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MRPS10 Antibody [NBP2-19409]

Immunocytochemistry/ Immunofluorescence: MRPS10 Antibody [NBP2-19409]

Immunocytochemistry/Immunofluorescence: MRPS10 Antibody [NBP2-19409] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.
MRPS10 Antibody

Immunohistochemistry: MRPS10 Antibody [NBP2-19409] -

Immunohistochemistry: MRPS10 Antibody [NBP2-19409] - MRPS10 antibody [N1C3] detects MRPS10 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: MRPS10 antibody [N1C3] (NBP2-19409) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for MRPS10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MRPS10

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [provided by RefSeq]

Alternate Names

28S ribosomal protein S10, mitochondrial, FLJ10567, mitochondrial 28S ribosomal protein S10, mitochondrial ribosomal protein S10, MRP-S10, PNAS-122, S10mt

Gene Symbol

MRPS10

UniProt

Additional MRPS10 Products

Product Documents for MRPS10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRPS10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.