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MRPS25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85151

Novus Biologicals, part of Bio-Techne
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NBP1-85151

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPS25 Antibody

Western Blot: MRPS25 Antibody [NBP1-85151]

Western Blot: MRPS25 Antibody [NBP1-85151]

Western Blot: MRPS25 Antibody [NBP1-85151] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: MRPS25 Antibody [NBP1-85151]

Immunocytochemistry/ Immunofluorescence: MRPS25 Antibody [NBP1-85151]

Immunocytochemistry/Immunofluorescence: MRPS25 Antibody [NBP1-85151] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: MRPS25 Antibody [NBP1-85151]

Immunohistochemistry-Paraffin: MRPS25 Antibody [NBP1-85151]

Immunohistochemistry-Paraffin: MRPS25 Antibody [NBP1-85151] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Applications for MRPS25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPS25

MRPS25, or Mitochondrial Ribosomal Protein S25, consists of a 173 amino acid isoform that is 20 kDa, and is involved in mitochondrial protein synthesis. The protein is not currently involved in any disease research. MRPS25 has been linked to the process of translation, and interacts with ICT1, DDX56, MME, KBTBD7, and SQSTM1.

Alternate Names

FLJ00023, mitochondrial 28S ribosomal protein S25, mitochondrial ribosomal protein S25, MRP-S2528S ribosomal protein S25, mitochondrial, RPMS25DKFZp313H0817, S25mt

Gene Symbol

MRPS25

Additional MRPS25 Products

Product Documents for MRPS25 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRPS25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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