MRPS27 Antibody (OTI1A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03888

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A2

Concentration

1 mg/ml

View all MRPS27 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRPS27 Antibody (OTI1A2)

Western Blot: MRPS27 Antibody (OTI1A2) [NBP2-03888]

Western Blot: MRPS27 Antibody (OTI1A2) [NBP2-03888]

Western Blot: MRPS27 Antibody (1A2) [NBP2-03888] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
Immunohistochemistry-Paraffin: MRPS27 Antibody (1A2) [NBP2-03888] Staining of paraffin-embedded Human lung tissue using anti-MRPS27 mouse monoclonal antibody.
Flow Cytometry: MRPS27 Antibody (OTI1A2) [NBP2-03888]

Flow Cytometry: MRPS27 Antibody (OTI1A2) [NBP2-03888]

Flow Cytometry: MRPS27 Antibody (1A2) [NBP2-03888] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.
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Applications for MRPS27 Antibody (OTI1A2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRPS27

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Alternate Names

FLJ21764, KIAA0264FLJ23348, mitochondrial, mitochondrial ribosomal protein S27, MRP-S27

Entrez Gene IDs

23107 (Human)

Gene Symbol

MRPS27

Additional MRPS27 Products

Product Documents for MRPS27 Antibody (OTI1A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MRPS27 Antibody (OTI1A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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