MSH2 Antibody (10G3-R)

Western Blot: MSH2 Antibody (10G3-R) [NBP3-32604]

Western Blot: MSH2 Antibody (10G3-R) [NBP3-32604] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32604) at 1/5,000 dilution.

Lane 1: RAW264.7 cell lysate, 10 ug/Lane
Lane 2: PC-12 cell lysate, 10 ug/Lane
Lane 3: A431 cell lysate, 10 ug/Lane
Lane 4: NIH-3T3 cell lysate, 10 ug/Lane
Lane 5: Mouse testis tissue lysate, 20 ug/Lane
Lane 6: Mouse heart tissue lysate, 20 ug/Lane

Predicted band size: 105 kDa
Observed band size: 105 kDa

Exposure time: 2 minutes;

5% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32604) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MSH2 Antibody (10G3-R) [NBP3-32604]

Immunohistochemistry: MSH2 Antibody (10G3-R) [NBP3-32604] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MSH2 antibody (NBP3-32604) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (10G3-R) [NBP3-32604]

Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (10G3-R) [NBP3-32604] - Immunocytochemistry analysis of Daudi cells labeling MSH2 with Mouse anti-MSH2 antibody (NBP3-32604) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MSH2 antibody (NBP3-32604) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.