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MSH2 Antibody (CL13764)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24574

Novus Biologicals, part of Bio-Techne
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NBP3-24574-100ul
NBP3-24574-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13764

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was designed to target the recombinant protein MSH2, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MSH2 Antibody (CL13764)

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13764) [NBP3-24574]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13764) [NBP3-24574]

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13764) [NBP3-24574]

Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.

Applications for MSH2 Antibody (CL13764)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH2

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (CL13764)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MSH2 Antibody (CL13764)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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