MSH2 Antibody (CL13764)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24574
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (95%), Rat (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL13764
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for MSH2 Antibody (CL13764)
Immunogen
This antibody was designed to target the recombinant protein MSH2, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for MSH2 Antibody (CL13764)
Immunohistochemistry-Paraffin: MSH2 Antibody (CL13764) [NBP3-24574]
Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: MSH2 Antibody (CL13764) [NBP3-24574]
Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: MSH2 Antibody (CL13764) [NBP3-24574]
Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.
Applications for MSH2 Antibody (CL13764)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MSH2
Long Name
MutationS Homolog 2
Alternate Names
COCA1, FCC1, HNPCC1, LCFS2
Gene Symbol
MSH2
Additional MSH2 Products
Product Documents for MSH2 Antibody (CL13764)
Product Specific Notices for MSH2 Antibody (CL13764)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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