MSH2 Antibody (rMSH2/6548)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07210

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Canine, Feline

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # rMSH2/6548

Concentration

0.2 mg/ml

View all MSH2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MSH2 protein (Uniprot: IDP43246)

Localization

Nuclear

Marker

DNA Mismatch Repair Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08956)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MSH2 Antibody (rMSH2/6548)

Western Blot: MSH2 Antibody (rMSH2/6548) [NBP3-07210]

Western Blot: MSH2 Antibody (rMSH2/6548) [NBP3-07210]

Western Blot: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Western blot analysis of HCT116 cell lysate using MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2/6548).
Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210]

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210]

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Formalin-fixed, paraffin-embedded dog lymph node stained with MSH2 Antibody (rMSH2/6548).
Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210]

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210]

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Formalin-fixed, paraffin-embedded human colon stained with MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2/6548).
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Applications for MSH2 Antibody (rMSH2/6548)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MSH2

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (rMSH2/6548)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSH2 Antibody (rMSH2/6548)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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