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MSH3 Antibody (CL14064)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24588

Novus Biologicals, part of Bio-Techne
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NBP3-24588-100ul
NBP3-24588-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14064

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was designed to target the recombinant protein MSH3, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MSH3 Antibody (CL14064)

Immunohistochemistry-Paraffin: MSH3 Antibody (CL14064) [NBP3-24588]

Staining of human endometrium shows moderate nuclear positivity in glandular and in stromal cells.

Western Blot: MSH3 Antibody (CL14064) [NBP3-24588]

Western blot analysis in human cell line U-251 MG.

Immunohistochemistry-Paraffin: MSH3 Antibody (CL14064) [NBP3-24588]

Staining of human cervical cancer shows strong nuclear positivity in tumor cells.

Applications for MSH3 Antibody (CL14064)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH3

The function of MSH3 is unknown. It is probably a DNA-repair protein. Defects in MSH3 are a cause of susceptibility to endometrial cancer.

Alternate Names

Divergent upstream protein, DNA mismatch repair protein Msh3, DUC1, DUG, DUPMGC163306, hMSH3, Mismatch repair protein 1, MRP1MGC163308, mutS (E. coli) homolog 3, mutS homolog 3 (E. coli)

Gene Symbol

MSH3

Additional MSH3 Products

Product Documents for MSH3 Antibody (CL14064)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MSH3 Antibody (CL14064)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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