MSH5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20946

Novus Biologicals, part of Bio-Techne
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NBP1-20946
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MSH5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence GDLHCEIRDQET corresponding to internal region according to NP_751897.1, NP_002432.1.

Epitope

internal region

Specificity

This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MSH5 Antibody

Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946]

Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946]

Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946]

Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946]

Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MSH5 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.75 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.75 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 38 kDa band in human testis, tonsil and thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9 kDa band according to the smallest reported isoform NP_002432.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human testis shows nuclear staining in spermatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MSH5

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair or meiotic recombination processes. This protein is similar to a Saccharomyces cerevisiae protein that participates in meiotic segregation fidelity and crossing-over. This protein forms heterooligomers with another member of this family, mutS homolog 4. Alternative splicing results in four transcript variants encoding three different isoforms.

Alternate Names

DKFZp434C1615, G7, hMSH5, MGC2939, mutS (E. coli) homolog 5, mutS homolog 5 (E. coli), mutS protein homolog 5, MUTSH5, NG23

Entrez Gene IDs

4439 (Human); 17687 (Mouse); 294252 (Rat)

Gene Symbol

MSH5

UniProt

NP_079535.3 (Human), NP_751897.1 (Human), NP_002432.1 (Human)

Additional MSH5 Products

Product Documents for MSH5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MSH5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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