MSH6 Antibody (035)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89265

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Ferret, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 035

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MSH6 Antibodies »

Product Summary for MSH6 Antibody (035)

Immunogen

This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human MSH6.

Reactivity Notes

Cross reactivity in IHC:
Cynomolgus, Mouse, Rat, Ferret

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MSH6 Antibody (035)

Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (035) [NBP2-89265]

Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (035) [NBP2-89265]

Immunocytochemistry/Immunofluorescence: MSH6 Antibody (035) [NBP2-89265] - Staining of MSH6 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MSH6 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]

Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]

Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in Ferret kidney with rabbit monoclonal antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]

Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265]

Immunohistochemistry-Paraffin: MSH6 Antibody (035) [NBP2-89265] - Staining MSH6 in human rectal cancer with rabbit monoclonal antibody (1:200).
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Applications for MSH6 Antibody (035)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody (035)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSH6 Antibody (035)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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