MSH6 Antibody (MSH6/3086) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79846

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MSH6/3086

Format

Azide and BSA Free

Concentration

1 mg/ml

View all MSH6 Antibodies »

Product Summary for MSH6 Antibody (MSH6/3086) - Azide and BSA Free

Immunogen

Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary) (Uniprot: P52701)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79712).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MSH6 Antibody (MSH6/3086) - Azide and BSA Free

Western Blot: MSH6 Antibody (MSH6/3086)Azide and BSA Free [NBP2-79846]

Western Blot: MSH6 Antibody (MSH6/3086)Azide and BSA Free [NBP2-79846]

Western Blot: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846] - Western Blot Analysis of HCT116 cell lysate using MSH6 Antibody (MSH6/3086).
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846]

Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846]

Immunocytochemistry/Immunofluorescence: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846] - Immunofluorescence staining of PFA-fixed MCF-7 cells with MSH6 Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with RedDot (Red).
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846]

Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846]

Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) - Azide and BSA Free [NBP2-79846] - Formalin-fixed, paraffin-embedded human Small Intestine stained with MSH6 Antibody (MSH6/3086).
View 3 more images

Applications for MSH6 Antibody (MSH6/3086) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody (MSH6/3086) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSH6 Antibody (MSH6/3086) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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