Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all MSH6 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human MutS homolog 6 using the numbering given in entry NP_000170.1 (GeneID 2956).

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MSH6 Antibody

Western Blot: MSH6 Antibody [NB100-328]

Western Blot: MSH6 Antibody [NB100-328]

Western Blot: MSH6 Antibody [NB100-328] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MSH6 antibody NB100-328 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-328]

Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-328]

Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-328] - Section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-MSH6 used at a dilution of 1:500 (2ug/ml). Detection: DAB

Applications for MSH6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:250 - 1:1000

Immunohistochemistry-Paraffin

1:250 - 1:1000

Western Blot

1:1000-1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Entrez Gene IDs

2956 (Human)

Gene Symbol

MSH6

UniProt

NP_000170.1 (Human)

Additional MSH6 Products

Product Documents for MSH6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MSH6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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