MSK1/RPS6KA5 Antibody (1G7)

Novus Biologicals, part of Bio-Techne | Catalog # H00009252-M11

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MSK1 Antibodies »

Product Specifications

Immunogen

RPS6KA5 (AAH17187.1, 372 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FQGYSFVAPSILFKRNAAVIDPLQFHMGVERPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKSNQAFAVKIISKRMEANTQKEIT

Specificity

Reacts with ribosomal protein S6 kinase, 90kDa, polypeptide 5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MSK1/RPS6KA5 Antibody (1G7)

Immunocytochemistry/ Immunofluorescence: MSK1/RPS6KA5 Antibody (1G7) [H00009252-M11]

Immunocytochemistry/ Immunofluorescence: MSK1/RPS6KA5 Antibody (1G7) [H00009252-M11]

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 Antibody (1G7) [H00009252-M11] - Analysis of monoclonal antibody to RPS6KA5 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: MSK1/RPS6KA5 Antibody (1G7) [H00009252-M11] - Detection limit for recombinant GST tagged RPS6KA5 is 0.3 ng/ml as a capture antibody.

Applications for MSK1/RPS6KA5 Antibody (1G7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MSK1

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Alternate Names

RPS6KA5

Entrez Gene IDs

9252 (Human)

Gene Symbol

RPS6KA5

UniProt

AAH17187 (Human)

Additional MSK1 Products

Product Documents for MSK1/RPS6KA5 Antibody (1G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSK1/RPS6KA5 Antibody (1G7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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