MSK1/RPS6KA5 [p Ser376] Antibody (2F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26393

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26393-100ul
NBP3-26393-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MSK1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human MSK1/RPS6KA5 [p Ser376] [UniProt O75582]

Modification

p Ser 376

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MSK1/RPS6KA5 [p Ser376] Antibody (2F3)

MSK1/RPS6KA5 [p Ser376] Antibody (2F3)

Immunohistochemistry: MSK1/RPS6KA5 [p Ser376] Antibody (2F3) [NBP3-26393] -

Immunohistochemistry: MSK1/RPS6KA5 [p Ser376] Antibody (2F3) [NBP3-26393] - Image of MSK1/RPS6KA5 [p Ser376] Antibody (2F3) diluted at 1:100 and staining in paraffin-embedded human bladder cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Applications for MSK1/RPS6KA5 [p Ser376] Antibody (2F3)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MSK1

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Alternate Names

RPS6KA5

Gene Symbol

RPS6KA5

Additional MSK1 Products

Product Documents for MSK1/RPS6KA5 [p Ser376] Antibody (2F3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MSK1/RPS6KA5 [p Ser376] Antibody (2F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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