MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07556

Novus Biologicals, part of Bio-Techne
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NBP3-07556-20ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-RPS6KA5-1A8

Concentration

0.2 mg/ml

View all MSK1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MSK1/RPS6KA5 protein (Uniprot: O75582)

Reactivity Notes

Predicted to react with Mouse and Rat.

Localization

Nucleus, Cytoplasm. Predominantly nuclear. Partially cytoplasmic.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08282)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8)

Immunocytochemistry/ Immunofluorescence: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556]

Immunocytochemistry/ Immunofluorescence: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556]

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556] - Immunofluorescence Analysis of HeLa cells using MSK1/RPS6KA5 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556]

Flow Cytometry: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556]

Flow Cytometry: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MSK1/RPS6KA5 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).
Protein Array: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556]

Protein Array: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556]

Protein Array: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-07556] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MSK1/RPS6KA5 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8).

Applications for MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MSK1

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Alternate Names

RPS6KA5

Gene Symbol

RPS6KA5

UniProt

O75582

Additional MSK1 Products

Product Documents for MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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