MSK1/RPS6KA5 [p Ser376] Antibody (SR2276)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21542

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-21542-100ul
NBP3-21542-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2276

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MSK1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-MSK1/RPS6KA5 [Ser376] (Uniprot #: O75582)

Modification

p Ser 376

Specificity

Detects endogenous levels of phospho-MSK1/RPS6KA5 [Ser376]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MSK1/RPS6KA5 [p Ser376] Antibody (SR2276)

Western Blot: MSK1/RPS6KA5 [p Ser 376] Antibody (SR2276) [NBP3-21542] -

Western Blot: MSK1/RPS6KA5 [p Ser 376] Antibody (SR2276) [NBP3-21542] - Western blot analysis using NBP3-21542 in HEK293 cell lysate treated with EGF.

Immunohistochemistry: MSK1/RPS6KA5 [p Ser 376] Antibody (SR2276) [NBP3-21542] -

Immunohistochemistry: MSK1/RPS6KA5 [p Ser 376] Antibody (SR2276) [NBP3-21542] - Immunohistochemical analysis of paraffin-embedded human lung cancer, using NBP3-21542.

Applications for MSK1/RPS6KA5 [p Ser376] Antibody (SR2276)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSK1

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Alternate Names

RPS6KA5

Gene Symbol

RPS6KA5

Additional MSK1 Products

Product Documents for MSK1/RPS6KA5 [p Ser376] Antibody (SR2276)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MSK1/RPS6KA5 [p Ser376] Antibody (SR2276)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.