MSK1/RPS6KA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82618

Novus Biologicals, part of Bio-Techne
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NBP1-82618
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MSK1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEKLFQGYSFVAPSILFKRNAAVIDPLQFHMGVERPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MSK1/RPS6KA5 Antibody

Western Blot: MSK1/RPS6KA5 Antibody [NBP1-82618]

Western Blot: MSK1/RPS6KA5 Antibody [NBP1-82618]

Western Blot: MSK1/RPS6KA5 Antibody [NBP1-82618] - Analysis in human cell line SK-BR-3.
Immunohistochemistry-Paraffin: MSK1/RPS6KA5 Antibody [NBP1-82618]

Immunohistochemistry-Paraffin: MSK1/RPS6KA5 Antibody [NBP1-82618]

Immunohistochemistry-Paraffin: MSK1/RPS6KA5 Antibody [NBP1-82618] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: MSK1/RPS6KA5 Antibody [NBP1-82618]

Immunohistochemistry-Paraffin: MSK1/RPS6KA5 Antibody [NBP1-82618]

Immunohistochemistry-Paraffin: MSK1/RPS6KA5 Antibody [NBP1-82618] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.
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Applications for MSK1/RPS6KA5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSK1

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Long Name

Mitogen- and Stress-activated Protein Kinase 1

Alternate Names

RPS6KA5

Gene Symbol

RPS6KA5

Additional MSK1 Products

Product Documents for MSK1/RPS6KA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MSK1/RPS6KA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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