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MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33136

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-33136

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE46-64

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human MST1/2 protein. (Uniprot: Q13043)

Modification

p Thr183, p Thr180

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

Western Blot: MST1/2 [p Thr180, p Thr183] Antibody (JE46-64) [NBP3-33136]

Western Blot: MST1/2 [p Thr180, p Thr183] Antibody (JE46-64) [NBP3-33136] - Western blot analysis of MST1/2 on different lysates with Rabbit anti-MST1/2 antibody (NBP3-33136) at 1/1,000 dilution.

Lane 1: Ramos cell lysate
Lane 2: Ramos treated with 1uM Staurosporine for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33136) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MST1/2

Alternate Names

KRS1, KRS2, Mammalian STE20-like protein kinase 1, Mammalian STE20-like protein kinase 2, mammalian sterile 20-like 1, serine/threonine-protein kinase 3, serine/threonine-protein kinase 4, Serine/threonine-protein kinase Krs-1, YSK3

Gene Symbol

STK3

Additional MST1/2 Products

Product Documents for MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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