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MST2/STK3 Antibody (4F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00006788-M13

Novus Biologicals, part of Bio-Techne
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H00006788-M13

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

STK3 (AAH10640.1, 311 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKV

Specificity

STK3 - serine/threonine kinase 3 (STE20 homolog, yeast) (4F7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MST2/STK3 Antibody (4F7)

Western Blot: MST2/STK3 Antibody (4F7) [H00006788-M13]

Western Blot: MST2/STK3 Antibody (4F7) [H00006788-M13]

Western Blot: MST2/STK3 Antibody (4F7) [H00006788-M13] - Analysis of STK3 expression in HepG2 (Cat # L019V1).
Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody (4F7) [H00006788-M13]

Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody (4F7) [H00006788-M13]

Immunocytochemistry/Immunofluorescence: MST2/STK3 Antibody (4F7) [H00006788-M13] - Analysis of monoclonal antibody to STK3 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: MST2/STK3 Antibody (4F7) [H00006788-M13]

Western Blot: MST2/STK3 Antibody (4F7) [H00006788-M13]

Western Blot: MST2/STK3 Antibody (4F7) [H00006788-M13] - Analysis of STK3 expression in transfected 293T cell line by STK3 monoclonal antibody (M13), clone 4F7. Lane 1: STK3 transfected lysatE (56.3 KDa). Lane 2: Non-transfected lysate.

Applications for MST2/STK3 Antibody (4F7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]

Alternate Names

KRS1, Mess1, MST3, STK3

Entrez Gene IDs

6788 (Human)

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody (4F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MST2/STK3 Antibody (4F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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