MST2/STK3 Antibody (5J1I4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16737

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16737-100ul
NBP3-16737-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J1I4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MST2/STK3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MST2/STK3 (Q13188). MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVME

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MST2/STK3 Antibody (5J1I4)

Western Blot: MST2/STK3 Antibody (5J1I4) [NBP3-16737]

Western Blot: MST2/STK3 Antibody (5J1I4) [NBP3-16737]

Western Blot: MST2/STK3 Antibody (5J1I4) [NBP3-16737] - Western blot analysis of extracts of various cell lines, using MST2/STK3 Rabbit mAb (NBP3-16737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody (5J1I4) [NBP3-16737]

Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody (5J1I4) [NBP3-16737]

Immunocytochemistry/Immunofluorescence: MST2/STK3 Antibody (5J1I4) [NBP3-16737] - Immunofluorescence analysis of NIH-3T3 cells using MST2/STK3 Rabbit mAb (NBP3-16737) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for MST2/STK3 Antibody (5J1I4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody (5J1I4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MST2/STK3 Antibody (5J1I4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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