MST2/STK3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24997

Novus Biologicals, part of Bio-Techne
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NBP3-24997
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MST2/STK3 Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein MST2/STK3 using the following amino acid sequence: DLITEAMEIKAKRHEEQQRELEEEEENS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MST2/STK3 Antibody

MST2/STK3 Antibody Western Blot: MST2/STK3 Antibody [NBP3-24997]

Western Blot: MST2/STK3 Antibody [NBP3-24997]

Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10
Lane 2: RT-4
Lane 3: U-251MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
MST2/STK3 Antibody Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP3-24997]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP3-24997]

Staining of human ovary shows cytoplasmic positivity in follicle cells.

Applications for MST2/STK3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MST2/STK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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