MT-ND1 Antibody (JE34-93)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32613
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE34-93
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human MT-ND1 aa 23-67 / 318. (Uniprot: P03886)
Localization
Mitochondrion inner membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MT-ND1 Antibody (JE34-93)
Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613]
Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613] - Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (NBP3-32613) at 1/1,000 dilution.Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 36 kDa
Observed band size: 37 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32613) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for MT-ND1 Antibody (JE34-93)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MT-ND1
Alternate Names
EC 1.6.5.3, mitochondrially encoded NADH dehydrogenase 1, MTND1, NAD1, NADH dehydrogenase subunit 1, NADH dehydrogenase, subunit 1 (complex I), NADH1, ND1NADH dehydrogenase 1
Gene Symbol
ND1
Additional MT-ND1 Products
Product Documents for MT-ND1 Antibody (JE34-93)
Product Specific Notices for MT-ND1 Antibody (JE34-93)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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