MTA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56483

Novus Biologicals, part of Bio-Techne
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NB100-56483
NB100-56483SS
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all MTA2 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 650-700 of human MTA2 was used as the immunogen (NP_004730).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Dog (88%), Bovine (88%) and Xenopus (76%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTA2 Antibody

Western Blot: MTA2 Antibody [NB100-56483]

Western Blot: MTA2 Antibody [NB100-56483]

Western Blot: MTA2 Antibody [NB100-56483] - Lysate from Jurkat cells, in the 1) absence and 2) presence of immunizing peptide, probed with this antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: MTA2 Antibody [NB100-56483]

Immunocytochemistry/ Immunofluorescence: MTA2 Antibody [NB100-56483]

Immunocytochemistry/Immunofluorescence: MTA2 Antibody [NB100-56483] - This antibody was tested in HEK293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: MTA2 Antibody [NB100-56483]

Immunohistochemistry: MTA2 Antibody [NB100-56483]

Immunohistochemistry: MTA2 Antibody [NB100-56483] - Analysis of Human Testis using this antibody at 10 ug/ml.

Applications for MTA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTA2

Gene Map Locus: 11q12-q13.1 (human) MTA2, a homologue of human MTA1 and MTA3, is a member of highly conserved MTA gene family. It is a component of NuRD, an ATP-dependent nucleosome remodeling and histone deacetylase complex. MTA2 is a nuclear protein that interacts with HDAC1 and HDAC2 and has a functional role in chromatin remodeling and deacetylase activity. MTA2 specifically interacts with p53 and represses p53-dependant transcriptional activation, thereby regulating p53-mediated cell growth arrest and apoptosis. It also inhibits the transcriptional activity of Estrogen receptor-alpha. Northern Blot analysis detected a ubiquitous expression of MTA2.

Alternate Names

DKFZp686F2281, metastasis associated 1 family, member 2, metastasis -associated gene 1-like 1, metastasis associated gene family, member 2, Metastasis-associated 1-like 1, metastasis-associated protein 2, metastasis-associated protein MTA2, MTA1-L1 protein, MTA1L1MTA1-L1, p53 target protein in deacetylase complex, PID

Entrez Gene IDs

9219 (Human); 23942 (Mouse); 361724 (Rat)

Gene Symbol

MTA2

UniProt

O94776 (Human)

Additional MTA2 Products

Product Documents for MTA2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MTA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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