MTAP Antibody (MTAP/1813) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75731

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MTAP/1813

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all MTAP Antibodies »

Product Specifications

Immunogen

Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary) (Uniprot: Q13126)

Localization

Cytoplasmic

Specificity

Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Marker

Tumor Suppressor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75730).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MTAP Antibody (MTAP/1813) - Azide and BSA Free

Western Blot: MTAP Antibody (MTAP/1813)Azide and BSA Free [NBP2-75731]

Western Blot: MTAP Antibody (MTAP/1813)Azide and BSA Free [NBP2-75731]

Western Blot: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731] - Western Blot Analysis of Human HeLa, A431, HePG2 and HAP1 cell lysate using MTAP Mouse Monoclonal Antibody (MTAP/1813).
Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731]

Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731]

Immunohistochemistry-Paraffin: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731] - Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Mouse Monoclonal Antibody (MTAP/1813).
Protein Array: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731]

Protein Array: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731]

Protein Array: MTAP Antibody (MTAP/1813) - Azide and BSA Free [NBP2-75731] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications for MTAP Antibody (MTAP/1813) - Azide and BSA Free

Application
Recommended Usage

ELISA

2 - 4 ug/mL

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MTAP

MTAP encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

Long Name

5'-Methylthioadenosine Phosphorylase

Alternate Names

BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase

Gene Symbol

MTAP

Additional MTAP Products

Product Documents for MTAP Antibody (MTAP/1813) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MTAP Antibody (MTAP/1813) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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