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MTCO1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94458

Novus Biologicals, part of Bio-Techne
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NBP2-94458-0.02ml
NBP2-94458-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MTCO1 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human MTCO1 (YP_002124304.1). FSGYTLDQTYAKIHFTIMFIGVNLTFFPQHFLGLSGMPRRYSDYPDAYTTWNILSSVGSFISLTAVMLMIFMIWEAFASKRKVLMVEEPSMNLEWLYGCPP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTCO1 Antibody - Azide and BSA Free

Western Blot: MTCO1 AntibodyAzide and BSA Free [NBP2-94458]

Western Blot: MTCO1 AntibodyAzide and BSA Free [NBP2-94458]

Western Blot: MTCO1 Antibody [NBP2-94458] - Analysis of extracts of various cell lines, using MTCO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: MTCO1 Antibody - Azide and BSA Free [NBP2-94458]

Immunocytochemistry/ Immunofluorescence: MTCO1 Antibody - Azide and BSA Free [NBP2-94458]

Immunocytochemistry/Immunofluorescence: MTCO1 Antibody [NBP2-94458] - Analysis of PC-12 cells using MTCO1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MTCO1 Antibody - Azide and BSA Free [NBP2-94458]

Immunohistochemistry-Paraffin: MTCO1 Antibody - Azide and BSA Free [NBP2-94458]

Immunohistochemistry-Paraffin: MTCO1 Antibody [NBP2-94458] - Mouse heart using MTCO1 Rabbit pAb at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for MTCO1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTCO1

Alternate Names

COI, COX1, MTCO1, MT-COI, MT-COI mitochondrially encoded cytochrome c oxidase I

Gene Symbol

COX1

Additional MTCO1 Products

Product Documents for MTCO1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MTCO1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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